• Regulatory status:RUO
• Type:Native
• Source:Human seminal plasma
• Other names:PSA, Kallikrein-3, Gamma-seminoprotein,P-30 antigen, Semenogelase
• Species:Human

Type

Native

Description

Native protein isolated from pooled human seminal plasma, 237 AA, MW 26,089 kDa (calculated without glycosylation). Protein identity confirmed by LC-MS/MS (NCBI no. gi|511857).

Amino Acid Sequence

IVGGWECEKHSQPWQVLVASRGRAVCGGVLVHPQWVLTAAHCIRNKSVILLGRHSLFHPEDTGQVFQVSHSFPHPLYDMSLLKNRFLRPGDDSSHDLMLLRLSEPAELTDAVKVMDLPTQEPALGTTCYASGWGSIEPEEFLTPKKLQCVDLHVISNDVCAQVHPQKVTKFMLCAGRWTGGKSTCSGDSGGPLVCNGVLQGITSWGSEPCALPERPSLYTKVVHYRKWIKDTIVANP

Source

Human seminal plasma

Purity

>90%

SDS-PAGE Gel

SDS-PAGE analysis of Prostate-specific antigen native protein, 14% gel stained with Coomassie Brillant Blue G250

Endotoxin

< 1.0 EU/ug

Formulation

Filtered (0,4 μm) and lyophilized in 0,5 mg/mL in 0,05M phosphate buffer, 0,075M NaCl, pH 7,4.

Reconstitution

Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Applications

Western blotting, ELISA, Cell culture and/or animal studies, Immunological methods

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.

Quality Control Test

BCA to determine quantity of the protein.

SDS PAGE to determine purity of the protein.

LAL to determine quantity of endotoxin.

Note

All samples used for protein preparation were tested and found negative for HBsAg, HIV1,2, HCV, syphilis, aHBc, RRR. Since no test can absolutely assure the absence of all infectious agents, this product should be handled as a potential biohazard. This product is intended for research use only.