Type
Recombinant
Description
KGF/FGF-7 isoneof23 knownmembersoftheFGFfamily.Proteinsofthisfamilyplayacentralroleduringprenataldevelopment,postnatalgrowth,andtheregenerationofavarietyoftissues,bypromotingcellularproliferationanddifferentiation.KGF/FGF-7 isamitogenfactorspecificforepithelialcellsandkeratinocytes.KGF/FGF-7 signalsthroughFGFR2b.KGF/FGF-7 playsaroleinkidneyandlungdevelopment,aswellasinangiogenesisandwoundhealing.RecombinantHumanKGF/FGF-7 isan18.9 kDaproteinconsistingof163 aminoacidresidues.
AminoAcidSequence
CNDMTPEQMATNVNCSSPERHTRSYDYMEGGDIRVRRLFCRTQWYLRIDKRGKVKGTQEMKNNYNIMEIRTVAVGIVAIKGVESEFYLAMNKEGKLYAKKECNEDCNFKELILENHYNTYASAKWTHNGGEMFVALNQKGIPVRGKKTKKEQKTAHFLPMAIT
Source
E.coli
Purity
95%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyKGF-responsiveBaF3cellsis≤10 ng/ml,correspondingtoaspecificactivityof≥1×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein1×PBStoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
