Type

Recombinant

Description

Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsat,oradjacenttospecificresidues,oraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocesses,includingprenatalandpostnataldevelopment,reproduction,signaltransduction,theimmuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,frequentlyusedintheanalysisandproductionofproteins.Arg-CspecificallycleavesatthecarboxylsideofArginineresidues.Arg-Chasasulfhydrylrequirement;itisactivatedbydithiothreitol,cysteine,orothersulfhydryl-containingreagents.Thepresenceofcalciumionsisessential.Theenzymeisinhibitedbyoxidizingagentsandsulfhydrylreactants,andbyCo2+,Cu2+,Cd2+,andheavymetalions.RecombinantLysobacterEnzymogenesArg-Cisa26.8 kDaproteaseconsistingof252 aminoacidresiduesincludingaC-terminalHis-Tag.

AminoAcidSequence

GVGDIGSSDYCEKDIVCRVKPSAEFLSASKSVARMVFTPKTGYTGYCSGTLLNNSNSPKRQLFWSAAHCISTQKVANTLQTYWLYDATGCDNDTLSDKAVTLTGGATLLHSHATRDTLLLELKSAPPSGAYYAGWNSSAIATKGTAIEGIHHPSGDLKKYSLGSVTALSSTIDGKKPLTKVAWTTGVTEGGSSGSGLFTISSTSGYQLRGGLYGGTSYCSAPSDPDYYSQLDGVWSSIKTYFSPHHHHHHHH

Source

Hi-5Insectcells

Purity

98%

BiologicalActivity

Thereactionismeasuredasanincreaseinabsorbanceat253 nmresultingfromthehydrolysisofN-benzoyl-L-arginineethylester(BAEE).

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Forbestresultsuseonlyfreshlyreconstitutedprotease.Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Arg-CisactivatedbyDTT,Cysteine,orothersulfhydrylcontainingreagents.Thepresenceofcalciumionsisessential.Arg-CisinhibitedbyoxidizingagentsandsulfhydrylreactantsandbyCo2+,Cu2+,Cd2+,andheavymetal ions.

Storage/Expiration

 –20°C