Type

PolyclonalAntibody

Applications

Westernblotting

SourceofAntigen

E.coli

Hosts

Rabbit

Preparation

TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumansPLA2-IIE.

AminoAcidSequence

Theimmunizationantigen(15.8 kDa)isaproteincontaining139 AAofrecombinantHumansPLA2-IIE.N-TerminalHis-tag,16 extraAA(highlighted).

MRGSHHHHHHGMASHMNLVQFGVMIEKMTGKSALQYNDYGCYCGIGGSHWPVDQTDWCCHAHDCCYGRLEKLGCEPKLEKYLFSVSERGIFCAGRTTCQRLTCECDKRAALCFRRNLGTYNRKYAHYPNKLCTGPTPPC

TheaminoacidsequenceoftherecombinantHumanSecretedPhospholipaseA2-IIEis100%homologoustotheaminoacidsequenceoftheHumanSecretedPhospholipaseA2-IIEwithoutsignalsequence

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumansPLA2-IIE.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.

Reconstitution

Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.