Biovendor/sPLA2 IIA人、兔多克隆抗体/RD181055100/0.1 mg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/sPLA2 IIA人、兔多克隆抗体/RD181055100/0.1 mg

Biovendor/sPLA2 IIA人、兔多克隆抗体/RD181055100/0.1 mg

商品编号： RD181055100

品牌： Biovendor-Laboratorni

市场价： ¥5000.00

美元价： 3000.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:PolyclonalAntibody
Othernames:PhospholipaseA2membraneassociated,GIICsPLA2,GroupIIAphospholipaseA2,Non-pancreaticsecretoryphospholipaseA2,NPS-PLA2,Phosphatidylcholine2-acylhydrolase2A,PLA2G2A,PLA2B,PLA2L,RASF-A
Species:Human

Type

PolyclonalAntibody

Applications

Westernblotting

SourceofAntigen

E.coli

Hosts

Rabbit

Preparation

TheantibodywasraisedinrabbitsbyimmunizationwiththrecombinantHumansPLA2-IIA.

AminoAcidSequence

Theimmunizationantigen(15.8 kDa)isaproteincontaining140 AAofrecombinantHumansPLA2-IIA.N-TerminalHis-tag,16 extraAA(highlighted).

MRGSHHHHHHGMASHMNLVNFHRMIKLTTGKEAALSYGFYGCHCGVGGRGSPKDATDRCCVTHDCCYKRLEKRGCGTKFLSYKFSNSGSRITCAKQDSCRSQLCECDKAAATCFARNKTTYNKKYQYYSNKHCRGSTPRC

TheaminoacidsequenceoftherecombinantHumanSecretedPhospholipaseA2-IIAis100%homologoustotheaminoacidsequenceoftheHumanSecretedPhospholipaseA2-IIAwithoutsignalsequence

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumansPLA2-IIA.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.

Reconstitution

Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。