Biovendor/成骨细胞特异性因子2人、兔多克隆抗体/RD181045050/0.05 mg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/成骨细胞特异性因子2人、兔多克隆抗体/RD181045050/0.05 mg

Biovendor/成骨细胞特异性因子2人、兔多克隆抗体/RD181045050/0.05 mg

商品编号： RD181045050

品牌： Biovendor-Laboratorni

市场价： ¥5500.00

美元价： 3300.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:PolyclonalAntibody
Othernames:Periostin,PN,OSF-2,POSTN,Fasciclin-Ilike,OsteoblastSpecificFactor1(Pleiotrophin,PTN,Heparin-bindinggrowth-associatedmolecule,HB-GAM,Heparin-bindinggrowthfactor8,HBGF-8,OSF-1,Heparin-bindingneuriteoutgrowth-promotingfactor1,HBNF-1,Heparin-bindingbrainmitogen,HBBM),NCK
Species:Human

Type

PolyclonalAntibody

Applications

Westernblotting,Immunohistochemistry

AntibodiesApplications

SourceofAntigen

E.coli

Hosts

Rabbit

Preparation

TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHuman OSF-2.

AminoAcidSequence

Theimmunizationantigen(75 kDa)isaproteincontaining648 AAofrecombinantHumanOSF-2 and23 AA,N-TerminalHisTagandXa –cleavagesite(highlighted).Theantigencontainsaminoacidresidues22 to669 ofthehumanOSF-2 precursor.Theantigenincludesallfourfasciclindomainsof OSF-2.

MGHHHHHHHHHHSSGHIEGRHMRNNHYDKILAHSRIRGRDQGPNVCALQQILGTKKKYFSTCKNWYKKSICGQKTTVLYECCPGYMRMEGMKGCPAVLPIDHVYGTLGIVGATTTQRYSDASKLREEIEGKGSFTYFAPSNEAWDNLDSDIRRGLESNVNVELLNALHSHMINKRMLTKDLKNGMIIPSMYNNLGLFINHYPNGVVTVNCARIIHGNQIATNGVVHVIDRVLTQIGTSIQDFIEAEDDLSSFRAAAITSDILEALGRDGHFTLFAPTNEAFEKLPRGVLERFMGDKVASEALMKYHILNTLQCSESIMGGAVFETLEGNTIEIGCDGDSITVNGIKMVNKKDIVTNNGVIHLIDQVLIPDSAKQVIELAGKQQTTFTDLVAQLGLASALRPDGEYTLLAPVNNAFSDDTLSMVQRLLKLILQNHILKVKVGLNELYNGQILETIGGKQLRVFVYRTAVCIENSCMEKGSKQGRNGAIHIFREIIKPAEKSLHEKLKQDKRFSTFLSLLEAADLKELLTQPGDWTLFVPTNDAFKGMTSEEKEILIRDKNALQNIILYHLTPGVFIGKGFEPGVTNILKTTQGSKIFLKEVNDTLLVNELKSKESDIMTTNGVIHVVDKLLYPADTPVGNDQLLEILNKLIKYIQIKFVRGSTFKEIPVTVY

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHuman OSF-2.

AntibodyContent

0.05 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.

Reconstitution

Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。