Type
PolyclonalAntibody
Applications
Westernblotting
SourceofAntigen
E.coli
Hosts
Hen
Preparation
TheantibodywasraisedinchickenbyimmunizationwiththerecombinantHumanNeuroglobin.
AminoAcidSequence
Theimmunizationantigen(17 kDa)isaproteincontaining150 AAofrecombinantHumanNeuroglobinandoneextraAA,N-terminalmethionin(highlighted).
MERPEPELIRQSWRAVSRSPLEHGTVLFARLFALEPDLLPLFQYNCRQFSSPEDCLSSPEFLDHIRKVMLVIDAAVTNVEDLSSLEEYLASLGRKHRAVGVKLSSFSTVGESLLYMLEKCLGPAFTPATRAAWSQLYGAVVQAMSRGWDGE
SpeciesReactivity
Human.Dog.Rat.Notyettestedinotherspecies.
PurificationMethod
ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanNeuroglobin.
AntibodyContent
0.05 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.05 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.
