Type
MonoclonalAntibody
Applications
Westernblotting,Immunohistochemistry
AntibodiesApplications
SourceofAntigen
E.coli
Hosts
Mouse
Isotype
IgG2b
Clone
4B9
Preparation
TheantibodyisamousemonoclonalantibodyagainstrecombinantHumanMyostatinPropeptide.
AminoAcidSequence
Theimmunizationantigen(28 kDa)isaproteincontaining243 AAofrecombinantHumanMyostatinPropeptideand5 extraAA(highlighted).
MGNENSEQKENVEKEGLCNACTWRQNTKSSRIEAIKIQILSKLRLETAPNISKDVIRQLLPKAPPLRELIDQYDVQRDDSSDGSLEDDDYHATTETIITMPTESDFLMQVDGKPKCCFFKFSSKIQYNKVVKAQLWIYLRPVETPTTVFVQILRLIKPMKDGTRYTGIRSLKLDMNPGTGIWQSIDVKTVLQNWLKQPESNLGIEIKALDENGHDLAVTFPGPGEDGLNPFLEVKVTDTPKRSRRKLN
TheaminoacidsequenceoftherecombinantHumanMyostatinPropeptideis100%homologouswiththeaminoacidsequenceoftheHumanMyostatinPropeptidewithoutsignalingsequence
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
Affinitychromatographyonacolumnwithimmobilizedprotein G.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.
