Type

PolyclonalAntibody

Applications

Westernblotting

SourceofAntigen

E.coli

Hosts

Bovine

Preparation

TheantibodywasraisedinbovinebyimmunizationwiththerecombinantHumanMyostatin.

AminoAcidSequence

Theimmunizationantigen(16.7kDa)isaproteincontaining152AAofrecombinantHumanMyostatin.N-terminalHis-tagincludingthespacer43AA(highlighted).TheAAsequenceofthehumanmyostatinpartofthefusionproteiniscorrespondingtotheUniProtKB/Swiss-ProtentryO14793,AA267–375.

MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDPSSRSAVRSRRDFGLDCDEHSTESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGECEFVFLQKYPHTHLVHQANPRGSAGPCCTPTKMSPINMLYFNGKEQIIYGKIPAMVVDRCGCS

TheaminoacidsequenceoftherecombinantHumanMyostatinis100%homologoustotheaminoacidsequenceoftheHumanMyostatin.

SpeciesReactivity

Human

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanMyostatin.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.

Reconstitution

Add0.1mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat–20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat–80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody