Type
MonoclonalAntibody
Applications
Westernblotting,ELISA,Immunohistochemistry
AntibodiesApplications
SourceofAntigen
NSOmousemyelomacellline
Hosts
Mouse
Isotype
IgG1
Clone
LPR-02
Preparation
TheantibodyisamousemonoclonalantibodyagainstrecombinantHumanLeptinReceptor.TheHumanLeptinReceptorisarecombinantproteinproducedinmousemyelomacellline.DNAsequenceincludingtheextracellulardomainofLeptinReceptor(aminoacidresidues1 839)fusedtotheFcregionofhumanIgG(withIIEGRaddedattheaminoterminusand6 histidineresiduesaddedatthecarboxyterminus).
SpeciesReactivity
Human.Doesnotreactwithmouse.Notyettestedinotherspecies.
PurificationMethod
Affinitychromatographyonacolumnwithimmobilizedprotein G.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.