Type

MonoclonalAntibody

Applications

Westernblotting,ELISA,Dot,IS,Immunoprecipitation

Hosts

Mouse

Isotype

IgG2b

Clone

HIS.H8/EH158

Preparation

WB(withECL):1:1000–3000dilution(incubateforonehouratroomtemperature)IS:1:500–2000dilutionForbestresultswithotherassays(e.g.:Dot,ELISA,IP,etc),pleasedetermineoptimalworkingdilutionbytitration test.

PurificationMethod

ProteinA affinitychromatographyfrommouseascites fluid.

Formulation

1 mg/mLin10 mMPBS,pH7.2,with0.05%NaN_3.

Storage/Expiration

Storeat –20°Cforupto2 years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze-thawcycles.Storealiquotsat4°Cfor1–2 weeks.