Type
MonoclonalAntibody
Applications
Westernblotting
SourceofAntigen
E.coli
Hosts
Mouse
Isotype
IgG2A
Preparation
TheantibodyisamousemonoclonalantibodyagainstrecombinantHumanHerstatin.
AminoAcidSequence
Theimmunizationantigen(434 kDa)isaproteincontaining397 AAofrecombinantHumanHerstatinandoneextraAA,N-terminalmethionin(highlighted)
MTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARGTHSLPPRPAAVPVPLRMQPGPAHPVLSFLRPSWDLVSAFYSLPLAPLSPTSVPISPVSVGRGPDPDAHVAVDLSRYEG
SpeciesReactivity
Human.Notyettestedinotherspecies.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
WesternBlot-toconfirmspecifityoftheantibody
Note
Thisproductisforresearchuseonly.