Type
MonoclonalAntibody
Applications
Westernblotting,ELISA,Dot,IS
Hosts
Mouse
Isotype
IgG1
Clone
GA1R
Preparation
WB(withECL):1:1000–10,000 dilution(incubatefor1 houratroomtemperature)IS:1:500–2000dilutionForbestresultswithotherassays(e.g.:Dot,ELISA,etc),pleasedetermineoptimalworkingdilutionbytitration test
PurificationMethod
ProteinA affinitychromatographyfrommouseascites fluid
Formulation
1 mg/mLin10 mMPBS,pH7.2,with0.05%NaN3 Storeat –20°Cforupto2 years.Centrifugeafterfirstthawtomaximizeproductrecovery.Aliquottoavoidrepeatedfreeze-thawcycles.Storealiquotsat4°Cfor1–2 weeksStorage/Expiration