Type
MonoclonalAntibody
Applications
Westernblotting,Immunocytochemistry,ELISA,Immunofluorescence
SourceofAntigen
TheantibodywaspreparedagainstTritonX-100 humanspermextract.
Hosts
Mouse
Isotype
IgG1
Clone
Hs-3
Preparation
TheantibodyisamousemonoclonalantibodyagainstHumanClusterin.
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
AffinitychromatographyonacolumnwithimmobilizedproteinA-SepharosefollowedbyDEAE-chromatography.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2;15 mMSODIUM AZIDE.
Reconstitution
Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductfterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
SDSPAGE –todeterminepurityoftheantibody.
Note
Thisproductisforresearchuseonly.
