Type

MonoclonalAntibody

Applications

Westernblotting

SourceofAntigen

E.coli

Hosts

Mouse

Isotype

IgG2b

Clone

4B9

Preparation

TheantibodyisamousemonoclonalantibodyagainstrecombinantHumanProcathepsin K.

AminoAcidSequence

Theimmunizationantigen(36.7 kDa)isaproteincontaining314 aminoacidresiduesofrecombinantHumanProcathepsinK and12 additionalaminoacidresidues –HisTag(highlighted).TherecombinantHumanProcathepsinK iswithoutsignalpeptid(15 aminoacidresidues).

MRGSHHHHHHGSLYPEEILDTHWELWKKTHRKQYNNKVDEISRRLIWEKNLKYISIHNLEASLGVHTYELAMNHLGDMTSEEVVQKMTGLKVPLSHSRSNDTLYIPEWEGRAPDSVDYRKKGYVTPVKNQGQCGSCWAFSSVGALEGQLKKKTGKLLNLSPQNLVDCVSENDGCGGGYMTNAFQYVQKNRGIDSEDAYPYVGQEESCMYNPTGKAAKCRGYREIPEGNEKALKRAVARVGPVSVAIDASLTSFQFYSKGVYYDESCNSDNLNHAVLAVGYGIQKGNKHWIIKNSWGENWGNKGYILMARNKNNACGIANLASFPKM

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

Immunoaffinitychromatographyonacolumnwithimmobilizedprotein G.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2.AZIDEFREE.

Reconstitution

Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.