Type

PolyclonalAntibody

Applications

Westernblotting,ELISA

SourceofAntigen

E.coli

Hosts

Rabbit

Preparation

TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumanApolipoprotein D.

AminoAcidSequence

Theimmunizationantigen(19.82 kDa –calculated)isaproteincontaining174 AAofrecombinantHumanApolipoproteinD.C-terminalHis-tag,7 extraAA(highlighted).TheaminoacidsequenceofthisproductcorrespondstotheUniProtKB/Swiss-ProtentryP05090(HumanApoD).Followingmodificationsweremade:FouraminoacidexchangeswereintroducedatthesurfaceofApoD(Trp99His,Cys116Ser,Ile118Ser,Leu120Ser)toenhancethesolubilityoftherecombinantproteinandanotherthree(Leu23Pro,Pro133Val,Asn134Ala)tofacilitateitsgeneticmanipulation(highlighted).

FHLGKCPNPPVQENFDVNKYPGRWYEIEKIPTTFENGRCIQANYSLMENGKIKVLNQELRADGTVNQIEGEATPVNLTEPAKLEVKFSWFMPSAPYHILATDYENYALVYSCTSISQSFHVDFAWILARNVALPPETVDSLKNILTSNNIDVKKMTVTDQVNCPKLSAHHHHHH

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanApolipoprotein D.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE.

Reconstitution

Add0.2 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.