生物供应商/血管生成素样蛋白3人、兔多克隆抗体/RD181092100/0.1 mg-免疫在线蚂蚁淘旗下平台

商品详细生物供应商/血管生成素样蛋白3人、兔多克隆抗体/RD181092100/0.1 mg

生物供应商/血管生成素样蛋白3人、兔多克隆抗体/RD181092100/0.1 mg

商品编号： RD181092100

品牌： Biovendor-Laboratorni

市场价： ¥5000.00

美元价： 3000.00

产地：美国(厂家直采)

公司：

产品分类：多克隆抗体

公司分类： Polyclonal_antibody

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:PolyclonalAntibody
Othernames:Angiopoietin-relatedprotein3,Angiopoietin-5,ANG-5,ANGPT5,UNQ153/PRO179,ANGPTL-3
Species:Human

Type

PolyclonalAntibody

Applications

Westernblotting,ELISA

SourceofAntigen

E.coli

Hosts

Rabbit

Preparation

TheantibodywasraisedinrabbitsbyimmunizationwiththerecombinantHumanANGPTL3.

AminoAcidSequence

Theimmunizationantigen(26 kDa)isaproteincontaining223 AAofrecombinantHumanANGPTL3.N-TerminalHis-tag,16 extraAA(highlighted).

MRGSHHHHHHGMASHMSRIDQDNSSFDSLSPEPKSRFAMLDDVKILANGLLQLGHGLKDFVHKTKGQINDIFQKLNIFDQSFYDLSLQTSEIKEEEKELRRTTYKLQVKNEEVKNMSLELNSKLESLLEEKILLQQKVKYLEEQLTNLIQNQPETPEHPEVTSLKTFVEKQDNSIKDLLQTVEDQYKQLNQQHSQIKEIENQLRRTSIQEPTEISLSSKPRAP

TheaminoacidsequenceoftherecombinantHumanANGPTL3is100%homologoustotheaminoacidsequenceoftheHumanANGPTL3(AA17–223)withoutsignalsequence

SpeciesReactivity

Human.Notyettestedinotherspecies.

PurificationMethod

ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanANGPTL3.

AntibodyContent

0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)

Formulation

Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2,AZIDEFREE.

Reconstitution

Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.

Storage/Expiration

Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.

QualityControlTest

IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody

Note

Thisproductisforresearchuseonly.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。