Type
PolyclonalAntibody
Applications
Westernblotting,ELISA
SourceofAntigen
HEK293
Hosts
Goat
Preparation
TheantibodywasraisedingoatbyimmunizationwiththerecombinantHumanAdiponectin.
AminoAcidSequence
ETTTQGPGVLLPLPKGACTGWMAGIPGHPGHNGAPGRDGRDGTPGEKGEKGDPGLIGPKGDIGETGVPGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHIVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTNDYKDDDDK Glu1 toGln5 wereconfirmedbyN-terminalsequencing Human.Notyettestedinotherspecies. ImmunoaffinitychromatographyonacolumnwithimmobilizedrecombinantHumanAdiponectin. 0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard) Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDEFREE. Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation. Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below. Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C. IndirectELISA –todeterminetiteroftheantibody Thisproductisforresearchuseonly.SpeciesReactivity
PurificationMethod
AntibodyContent
Formulation
Reconstitution
Shipping
Storage/Expiration
QualityControlTest
SDSPAGE–todeterminepurityoftheantibodyNote