Type
MonoclonalAntibody
Applications
Westernblotting,ELISA
SourceofAntigen
Peptide
Hosts
Mouse
Isotype
IgG1
Clone
4H3
Preparation
Theantibodyisamousemonoclonalantibodyagainsthumanpeptide.
AminoAcidSequence
ThehumanpeptidewithaminoacidsequenceRASHLGLA(whichislocatedinC-terminalpartofthehuman ASP).
SpeciesReactivity
Human.Notyettestedinotherspecies.
PurificationMethod
Affinitychromatographyonacolumnwithimmobilizedprotein G.
AntibodyContent
0.1 mg(determinedbyBCAmethod,BSAwasusedasastandard)
Formulation
Theantibodyislyophilizedin0.05 Mphosphatebuffer,0.1 MNaCl,pH7.2. AZIDE FREE.
Reconstitution
Add0.1 mlofdeionizedwaterandletthelyophilizedpelletdissolvecompletely.Slightturbiditymayoccurafterreconstitution,whichdoesnotaffectactivityoftheantibody.Inthiscaseclarifythesolutionbycentrifugation.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Thelyophilizedantibodyremainsstableandfullyactiveuntiltheexpirydatewhenstoredat –20°C.Aliquottheproductafterreconstitutiontoavoidrepeatedfreezing/thawingcyclesandstorefrozenat –80°C.Reconstitutedantibodycanbestoredat4°Cforalimitedperiodoftime;itdoesnotshowdeclineinactivityafteroneweekat 4°C.
QualityControlTest
IndirectELISA –todeterminetiteroftheantibody
SDSPAGE–todeterminepurityoftheantibody
Note
Thisproductisforresearchuseonly.
