Type

Recombinant

Description

Total291AA,33,5 kDa(computed).TheAAsequence(14–291)isidenticaltoSwiss-Prot-P25311(AA18–295matureZinc-Alfa-2-Glycoprotein).THIRTEENextraaminoacidwerefusedwithN-terminus(highlighted).

AminoAcidSequence

PGDYKDDDDKPAGQENQDGRYSLTYIYTGLSKHVEDVPAFQALGSLNDLQFFRYNSKDRKSQPMGLWRQVEGMEDWKQDSQLQKAREDIFMETLKDIVEYYNDSNGSHVLQGRFGCEIENNRSSGAFWKYYYDGKDYIEFNKEIPAWVPFDPAAQITKQKWEAEPVYVQRAKAYLEEECPATLRKYLKYSKNILDRQDPPSVVVTSHQAPGEKKKLKCLAYDFYPGKIDVHWTRAGEVQEPELRGDVLHNGNGTYQSWVVVAVPPQDTAPYSCHVQHSSLAQPLVVPWEAS

Source

HEK293

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

12%SDS-PAGEseparationofHumanZA2G
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,10μg/lane
3.non-reducedandnon-heatedsample,10μg/lane

Endotoxin

<0.1 EU/ug

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA,Cellcultureand/oranimalstudies

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.