Type

Recombinant

Description

Proteases(alsocalledProteolyticEnzymes,Peptidases,orProteinases)areenzymesthathydrolyzetheamidebondswithinproteinsorpeptides.Mostproteasesactinaspecificmanner,hydrolyzingbondsat,oradjacentto,specificresidues,oraspecificsequenceofresiduescontainedwithinthesubstrateproteinorpeptide.ProteasesplayanimportantroleinmostdiseasesandBIOLOGicalprocesses,includingprenatalandpostnataldevelopment,reproduction,signaltransduction,immuneresponse,variousautoimmuneanddegenerativediseases,andcancer.Theyarealsoanimportantresearchtool,astheyarefrequentlyusedintheanalysisandproductionofproteins.Kex-2 cleavesatthecarboxylendoftherecognitionsequencesArg-Arg/XandLys-Arg/X.RecombinantYeastKex-2 isa60.4 kDaproteaseconsistingof558 aminoacidresidues.

AminoAcidSequence

LPVPAPPMDSSLLPVKEAEDKLSINDPLFERQWHLVNPSFPGSDINVLDLWYNNITGAGVVAAIVDDGLDYENEDLKDNFCAEGSWDFNDNTNLPKPRLSDDYHGTRCAGEIAAKKGNNFCGVGVGYNAKISGIRILSGDITTEDEAASLIYGLDVNDIYSCSWGPADDGRHLQGPSDLVKKALVKGVTEGRDSKGAIYVFASGNGGTRGDNCNYDGYTNSIYSITIGAIDHKDLHPPYSEGCSAVMAVTYSSGSGEYIHSSDINGRCSNSHGGTSAAAPLAAGVYTLLLEANPNLTWRDVQYLSILSAVGLEKNADGDWRDSAMGKKYSHRYGFGKIDAHKLIEMSKTWENVNAQTWFYLPTLYVSQSTNSTEETLESVITISEKSLQDANFKRIEHVTVTVDIDTEIRGTTTVDLISPAGIISNLGVVRPRDVSSEGFKDWTFMSVAHWGENGVGDWKIKVKTTENGHRIDFHSWRLKLFGESIDSSKTETFVFGNDKEEVEPAATESTVSQYSASSTSISISATSTSSISIGVETSAIPQTTTASTDPDSDPNTP

Source

(BTI-Tn-5B1-4)Hi-5Insectcells*

Purity

95%

BiologicalActivity

RecombinantKex-2 fromHigh-5 insectcellscontainsthesamespecificactivityandrecognitionsequencespecificityasyeastderivedKEX-2.1 milligramofrecombinantKEX-2 containsactivityequivalenttoatleast40 unitsofyeastderivedKEX-2. Cleavesatthecarboxylsideof K/R-R.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C