Type
Recombinant
Description
Wnt-3abelongstotheWntfamilyofsignalingproteinsthatplayakeyroleinmaintainingtheintegrityofembryonicandadulttissues.ExpressionofWnt-3aoccursprimarilyalongthedorsalmidlineacrossoverlappingregionsoftheCentralNervousSystem(CNS).Wnt-3asignalingisessentialforvariousmorphogeneticevents,includingembryonicpatterning,celldetermination,cellproliferation,CNSdevelopment,andcytoskeletalformation.Likeothermembersofthisfamily,Wnt-3acontainsahighlyconservedlipid-modified,cysteine-richdomainthatisessentialforcellsignaling.DuringabiochemicalprocesscalledthecanonicalWntpathway,Wntfamilymembersbindtoandactivate,seven-passtransmembranereceptorsoftheFrizzledfamily,ultimatelyleADIngtothedisruptionofβ-catenindegradation.Intracellularaccumulationofβ-cateninincreasestranslocationoftheproteinintothenucleus,whereitbindstoTCF/LEFtranscriptionfactorstopromotegeneexpression.LackofWntsignalingdisruptstranscriptionalactivationoftumorsuppressorgenes,andhasbeenshowntoresultinneoplastictransformation,oncogenesis,andhumandegenerativediseases.RecombinantMurineWnt-3aisamonomericglycoproteincontaining334 aminoacidresidues.Duetoglycosylation,theMurineWnt-3amigratesatanapparentmolecularweightofapproximately38.0–41.0 kDabySDS-PAGEanalysisundernon-reducingconditions.
AminoAcidSequence
SYPIWWSLAVGPQYSSLSTQPILCASIPGLVPKQLRFCRNYVEIMPSVAEGVKAGIQECQHQFRGRRWNCTTVSNSLAIFGPVLDKATRESAFVHAIASAGVAFAVTRSCAEGSAAICGCSSRLQGSPGEGWKWGGCSEDIEFGGMVSREFADARENRPDARSAMNRHNNEAGRQAIASHMHLKCKCHGLSGSCEVKTCWWSQPDFRTIGDFLKDKYDSASEMVVEKHRESRGWVETLRPRYTYFKVPTERDLVYYEASPNFCEPNPETGSFGTRDRTCNVSSHGIDGCDLLCCGRGHNARTERRREKCHCVFHWCCYVSCQECTRVYDVHTCK
Source
CellCulture
Purity
75%
BIOLOGicalActivity
TheED50wasdeterminedbyitsABIlitytoinducedalkalinephosphataseproductionbyCCL-226 cells.TheexpectedED50forthiseffectis0.4–0.6 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof≤0.1mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C