Type
Recombinant
Description
Wnt-1 isasecretedproteinthatsignalsthroughtheFrizzledfamilyofcellsurfacereceptors,andisrequiredfornormalembryonicdevelopment.Wnt-1 activationinducesacomplexsignalingcascadethatultimatelyleadstotheincreasedexpressionofoverfiftygenes.AnimportantcomponentofWnt-1 signalingisthestABIlization,andresultingaccumulation,oftheintracellularsignalingprotein,β-catenin.Wntsignalinginducesandmaintainsthetransformedphenotype,and,incertainembryoniccelllines,supportsself-renewalintheabsenceofsignificantdifferentiation.ElevatedlevelsofWntproteinsareassociatedwithtumOrigenesis,andarepresentinnumeroushumanbreastcancers.MaturehumanWnt-1 isaglycosylatedproteincontaining343 aminoacidresidues.RecombinantHumanWnt-1 isa38.4 kDa,non-glycosylatedproteincontaining343 aminoacidresidues.
AminoAcidSequence
ANSSGRWWGIVNVASSTNLLTDSKSLQLVLEPSLQLLSRKQRRLIRQNPGILHSVSGGLQSAVRECKWQFRNRRWNCPTAPGPHLFGKIVNRGCRETAFIFAITSAGVTHSVARSCSEGSIESCTCDYRRRGPGGPDWHWGGCSDNIDFGRLFGREFVDSGEKGRDLRFLMNLHNNEAGRTTVFSEMRQECKCHGMSGSCTVRTCWMRLPTLRAVGDVLRDRFDGASRVLYGNRGSNRASRAELLRLEPEDPAHKPPSPHDLVYFEKSPNFCTYSGRLGTAGTAGRACNSSSPALDGCELLCCGRGHRTRTQRVTERCNCTFHWCCHVSCRNCTHTRVLHECL
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbyitsabilitytoenhanceBMP-2 inducedalkalinephosphataseproductionbymurineATDC5cells.TheexpectedED50forthiseffectis1.5 –2.5 ng/mlinthepresenceof200 ng/mlofhuman BMP-2.
Storage/Expiration
–20°C
