Type

Recombinant

Description

Viral MIP-2 (vMIP-2) is a chemokine analog encoded by the human herpes virus, and has been shown to have antagonist activity towards several chemokine receptors. Recombinant Viral MIP-2 is a 7.9 kDa protein consisting of 70 amino acids, including the four highly conserved cysteine residues present in CC chemokines.

Amino Acid Sequence

LGASWHRPDKCCLGYQKRPLPQVLLSSWYPTSQLCSKPGVIFLTKRGRQVCADKSKDWVKKLMQQLPVTA

Source

E. coli

Purity

98%

Biological Activity

Determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0µg-10.0µg/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.

Endotoxin

Endotoxin level is <0.1 ng/μg of protein (<1EU/μg).

Reconstitution

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1–1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at –20°C to –80°C.

Storage/Expiration

 –20°C