Type
Recombinant
Description
VEGFisapotentgrowthandangiogeniccytokine.Itstimulatesproliferationandsurvivalofendothellialcells,andpromotesangiogenesisandvascularpermeABIlity.Expressedinvascularizedtissues,VEGFplaysaprominentroleinnormalandpathologicalangiogenesis.SubstantialevidenceimplicatesVEGFintheinductionoftumormetastasisandintra-ocularneovascularsyndromes.VEGFsignalsthroughthethreereceptors;fms-liketyrosinekinase(flt-1),KDRgeneproduct(themurinehomologofKDRistheflk-1 geneproduct)andtheflt4geneproduct.RecombinantRatVEGF165isa38.5 kDa,disulfide-linkedhomodimericproteinconsistingoftwo165 aminoacidpolypeptidechains.
AminoAcidSequence
MAPTTEGEQKAHEVVKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEALECVPTSESNVTMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPEKHCEPCSERRKHLFVQDPQTCKCSCKNTDSRCKARQLELNERTCRCDKPRR
Source
E.coli
Purity
98%
BIOLOGicalActivity
Determinedbythedose-dependentstimulationoftheproliferationofhumanumbilicalveinendothelialcells(HUVEC).TheexpectedED50is4.0–8.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
