Type

Recombinant

Description

VEGFisapotentgrowthandangiogeniccytokine.Itstimulatesproliferationandsurvivalofendothelialcells,andpromotesangiogenesisandvascularpermeABIlity.Expressedinvascularizedtissues,VEGFplaysaprominentroleinnormalandpathologicalangiogenesis.VEGFsignalsthroughthreereceptors;fms-liketyrosinekinase(flt-1),KDRgeneproduct(themurinehomologofKDRistheflk-1 geneproduct)andtheflt4geneproduct.Duetoitsincreasedacidity,VEGF121circulatesmorefreelythanotherVEGFforms,whichbindmoretightlywithvascularheparinsulfates.RecombinantHumanVEGF121isa28.4 kDa,disulfide-linkedhomodimericproteinconsistingoftwo121 aminoacidpolypeptidechains.

AminoAcidSequence

APMAEGGGQNHHEVVKFMDVYQRSYCHPIETLVDIFQEYPDEIEYIFKPSCVPLMRCGGCCNDEGLECVPTEESNITMQIMRIKPHQGQHIGEMSFLQHNKCECRPKKDRARQENCDKPRR

Source

E.coli

Purity

98%

BIOLOGicalActivity

Determinedbythedose-dependentstimulationoftheproliferationofhumanumbilicalveinendothelialcells(HUVEC)usingaconcentrationrangeof0.2–0.4 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C