Type
Recombinant
Description
VCAMisa110 kDa,cellsurfaceintegralmembraneglycoproteinthatbelongstotheIg-relatedsuperfamilyofadhesionmolecules.TheprimaryfunctionofVCAM-1 isthemediationofleukocyte-endothelialcelladhesionandsignaltransduction.VCAM-1 mayplayavitalroleinthedevelopmentofseveraldiseases,includingatherosclerosisandrheumatoidarthritis.ThehumanVCAM-1 genecodesfora715 aminoacidtransmembraneglycoproteincontaininga19 aminoacidcytoplasmicdomain,a22 aminoacidtransmembranedomain,anda674 aminoacidextracellulardomain.RecombinantHumanVCAM-1 isa74.1 kDaglycoproteincomprisingtheextracellulardomain(674 aminoacidresidues)ofVCAM-1. MonomericglycosylatedVCAM-1 migratesatanapparentmolecularweightofapproximately90.0 kDabySDS-PAGEanalysisunderreducingconditions.
AminoAcidSequence
FKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSP
Source
HEK293cells
Purity
97%
BIOLOGicalActivity
DeterminedbyitsABIlitytosupporttheadhesionofhumanU937cells.TheexpectedED50forthiseffectis0.8–1.0 μg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
