Type

Recombinant

Description

VAP-1 isatypeIImembranecelladhesionproteinbelongingtothecopper/topaquinoneoxidasefamily.Itisprimarilyexpressedonthehighendothelialvenulesofperipherallymphnodesandonhepaticendothelia.VAP-1 cancatalyzetheoxidativedeaminationoflowmolecularweightamines,andplaysanimportantroleinthemigrationoflymphocytestoinflamedtissue.InhibitionofVAP-1 canprotectagainstinflammation-relateddamagetocertaininjuredtissues.Additionally,VAP-1 canfunctionasasignificantprognosticMarkerforcertaincancersandcardiovasculardiseases.RecombinantHumanVAP-1 isamixtureofmonomericanddisulfide-linkedhomodimericformsofa737 aminoacidpolypeptide,correspondingtoaminoacids27 to763 oftheVAP-1 precursor.ThecalculatedmolecularweightofRecombinantHumanVAP-1 is81.8 kDa.

AminoAcidSequence

GRGGDGGEPSQLPHCPSVSPSAQPWTHPGQSQLFADLSREELTAVMRFLTQRLGPGLVDAAQARPSDNCVFSVELQLPPKAAALAHLDRGSPPPAREALAIVFFGRQPQPNVSELVVGPLPHPSYMRDVTVERHGGPLPYHRRPVLFQEYLDIDQMIFNRELPQASGLLHHCCFYKHRGRNLVTMTTAPRGLQSGDRATWFGLYYNISGAGFFLHHVGLELLVNHKALDPARWTIQKVFYQGRYYDSLAQLEAQFEAGLVNVVLIPDNGTGGSWSLKSPVPPGPAPPLQFYPQGPRFSVQGSRVASSLWTFSFGLGAFSGPRIFDVRFQGERLVYEISLQEALAIYGGNSPAAMTTRYVDGGFGMGKYTTPLTRGVDCPYLATYVDWHFLLESQAPKTIRDAFCVFEQNQGLPLRRHHSDLYSHYFGGLAETVLVVRSMSTLLNYDYVWDTVFHPSGAIEIRFYATGYISSAFLFGATGKYGNQVSEHTLGTVHTHSAHFKVDLDVAGLENWVWAEDMVFVPMAVPWSPEHQLQRLQVTRKLLEMEEQAAFLVGSATPRYLYLASNHSNKWGHPRGYRIQMLSFAGEPLPQNSSMARGFSWERYQLAVTQRKEEEPSSSSVFNQNDPWAPTVDFSDFINNETIAGKDLVAWVTAGFLHIPHAEDIPNTVTVGNGVGFFLRPYNFFDEDPSFYSADSIYFRGDQDAGACEVNPLACLPQAAACAPDLPAFSHGGFSHN

Source

ChineseHamsterOvaryCells(CHO)

Purity

>95%bySDS-PAGEgelandHPLCanalysis

BIOLOGicalActivity

MeasuredbyitsABIlitytoproducehydrogenperoxideduringtheoxidationofbenzylamine.Thespecificactivity>16pMoles/min/µgof VAP-1.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Formulation

Sterilefiltredthrougha0.2μm.Lyophilizedfrom10mMSodiumPhosphate,pH 7.8

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C

QualityControlTest

VerifiedbyUVSpectroscopyand/orSDS-PAGEgel.

Note

Thisproductisintendedforresearchuseonly.