Type
Native
Description
MW: 97 kDa (exp. SDS PAGE)
Source
Urine
SDS-PAGE Gel
SDS-PAGE analysis of Porcine Uromodulin native protein, 12% gel stained with Coomassie Brillant Blue G250
- M.W. marker – 14, 21, 31, 45, 66, 97 kDa
- reduced and heated sample, 5ug/lane
- non-reduced and non-heated sample, 5μg/lane
Endotoxin
< 1.0 EU/ug
Formulation
Filtered (0,4 μm) and lyophilized from 0.5mg/mL in deionized water
Reconstitution
Add deionized water to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
Applications
Western blotting, ELISA
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the lyophilized protein at –80 °C. Lyophilized protein remains stable until the expiry date when stored at –80 °C. Aliquot reconstituted protein to avoid repeated freezing/thawing cycles and store at –80 °C for long term storage. Reconstituted protein can be stored at 4 °C for a week.
Quality Control Test
BCA to determine quantity of the protein.
SDS PAGE to determine purity of the protein.
LAL to determine quantity of endotoxin.
