Type
Native
Description
Nativeproteinisolatedfromcanineurine,589 AA,MW:64,483 kDa(calculatedwithoutglycosylation).ProteinidentityconfirmedbyLC-MS/MS(NCBIno.gi|50950249).
AminoAcidSequence
RSCSECHSNATCMEDGMVTTCSCLVGFTGSGFECVDLDECAIPGAHNCSEGSSCMNTLGSYLCTCPDGFRLTPGLGCIDVDECSEPGLSRCHALATCINNKGNYSCVCPAGYRGDGQHCECSPGSCGPGLDCVPVGDALVCADPCQEHRILDEYWRSTEYGAGYTCDVGLNGWYRFTGPGGVRLAETCVPVLHCNTAAPMWLNGTHPTRDQGIVNRTACAHWRGHCCLWDASIQVKACAGGYYVYNLTETPECYLAYCTDPTSVLGTCEECSVEEDCKSHDGMWSCQCKQDFNVTDLFLLDRLECRPNDIKVSLSKCQLKSLGFEKVFMYLRDSQCSGFNERGDRDWVSVVTPARDGPCGTVMVRNETHATYSNTLYLADEIVIRDRNIKINFECSYPLDMKVSLETSLQPIVSSLNISVGGTGMFTVRMALFQTPDYTQPYQGSSVTLTTEAFLYVGTMLDGGDLSRFALLMTNCYATPSSNATDPLKYFIIQDRCPRTTDSTIQVVENGESPQGRFSVQMFRFAGNYDLVYLHCEVYLCDIINEKCKPTCSGTRFRSGGIIDQSRVLNLGPITRKNVQAVVSRAASS
Source
Canineurine
Purity
>95%
SDS-PAGEGel
SDS-PAGEanalysisofcanineuromodulinnativeprotein,12%gelstainedwithCoomassieBrillantBlueG250
- M.W.Marker –14,21,31,45,66,97 kDa
- reducedandheatedsample,2.5ug/lane
- non-reducedandnon-heatedsample,2.5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0.1 mg/mLindeionized water.
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.
Applications
Westernblotting,ELISA,Cellcultureand/oranimalstudies,Immunologicalmethods
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.
