Type
Recombinant
Description
TWEAKRbelongstotheTNFfamilyoftransmembraneproteins,andcontainsacytoplasmic“deathdomain”,whichcanactivateacell"s apoptoticmachinery.Itisexpressedinthespleen,thymus,peripheralbloodlymphocytes,colon,andsmallintestine.SignaltransductionbyTWEAKRcanbeactivatedbyeithermembrane-anchoredorsolubleTWEAK.RecombinantHumansolubleTWEAKRisa53 aminoacidpolypeptide(5.6 kDa)comprisingtheentireextracellulardomainofthefull-lengthTWEAKRprotein.
AminoAcidSequence
EQAPGTAPCSRGSSWSADLDKCMDCASCRARPHSDFCLGCAAAPPAPFRLLWP
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibitTWEAK-inducedweakcelldeathofHT29cells.TheexpectedED50forthiseffectis1.0–3.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
