Type
Recombinant
Description
TwistedGastrulationProtein(TSG)isasecretedBMP-bindingproteinthatisstructurallyrelatedtotheBMPantagoNISTsChordinandNoggin.TSGcaninhibitBMPactivitybybindingdirectlytoBMPproteins,andcanacteitherasaBMP-4 agonistorantagonist(dependingonthespecificbiochemicalenvironment)bybindingtotheBMP-4/Chordincomplex.RecombinantHumanTSGisa198 aminoacid,22.2 kDaproteincontainingtheBMP/TGF-βbindingportionofthefulllengthTSGprotein.
AminoAcidSequence
CNKALCASDVSKCLIQELCQCRPGEGNCSCCKECMLCLGALWDECCDCVGMCNPRNYSDTPPTSKSTVEELHEPIPSLFRALTEGDTQLNWNIVSFPVAEELSHHENLVSFLETVNQPHHQNVSVPSNNVHAPYSSDKEHMCTVVYFDDCMSIHQCKISCESMGASKYRWFHNACCECIGPECIDYGSKTVKCMNCMF
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoneutralizeBMP-6 inducedalkalinephosphataseproductionbyATDCchondrogeniccells.TheexpectedED50forthiseffectis2.0 µg/mlof TSG.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
