Type
Recombinant
Description
TPOisalineage-specificgrowthfactorproducedintheliver,kidneyandskeletalmuscle.Itstimulatestheproliferationandmaturationofmegakaryocytes,andpromotesincreasedcirculatinglevelsofplateletsinvivo.TPOsignalsthroughthec-mplreceptor,andactsasanimportantregulatorofcirculatingplatelets.HumanandmurineTPOexhibitcross-speciesreactivity.RecombinantRatTPOisafullyBIOLOGicallyactive174 aminoacidpolypeptide(18.7 kDa),whichcontainstheerythropoietin-likedomainofthefulllengthTPOprotein.
AminoAcidSequence
SPVPPACDPRLLNKLLRDSYLLHSRLSQCPDVNPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPPQGRTTAHKDPSALFLSLQQLLRGKVRFLLLVEGPALCVRRTLPTTAVPSRTSQLLTLNKF
Source
E.coli
Purity
98%
BiologicalActivity
DeterminedbyitsABIlitytostimulatetheproliferationofhumanMO7ecells.TheexpectedED50is≤0.2 ng/ml,correspondingtoaspecificactivityof≥5×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
