Type
Recombinant
Description
TPOisalineage-specificgrowthfactorproducedintheliver,kidneyandskeletalmuscle.Itstimulatestheproliferationandmaturationofmegakaryocytes,andpromotesincreasedcirculatinglevelsofplateletsinvivo.TPOsignalsthroughthec-mplreceptor,andactsasanimportantregulatorofcirculatingplatelets.HumanandmurineTPOexhibitcross-speciesreactivity.RecombinantMurineTPOisafullyBIOLOGicallyactive174 aminoacidpolypeptide(18.7 kDa),whichcontainstheerythropoietin-likedomainofthefulllengthTPOprotein.
AminoAcidSequence
SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKF
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofhumanMO7ecellswasfoundtobe≤1.0 ng/ml,correspondingtoaspecificactivityof≥1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof≤0.5 mg/ml.Slowtodissolve.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
