Type
Recombinant
Description
TPOisalineage-specificgrowthfactorproducedintheliver,kidneyandskeletalmuscle.Itstimulatestheproliferationandmaturationofmegakaryocytes,andpromotesincreasedcirculatinglevelsofplateletsinvivo.TPOsignalsthroughthec-mplreceptor,andactsasanimportantregulatorofcirculatingplatelets.HumanandmurineTPOexhibitcross-speciesreactivity.ThehumanTPOgeneencodesfora353 aminoacidglycoprotein,whichcontainsa21 aminoacidsignalsequence,a15 aminoaciderythropoietin-likedomain,andahighlyglycosylated179 aminoacidC-terminaldomain.RecombinantHumanTPOisafullyBIOLOGicallyactive174 aminoacidpolypeptide(18.6 kDa),whichcontainstheerythropoietin-likedomainofthefulllengthTPOprotein.
AminoAcidSequence
SPAPPACDLRVLSKLLRDSHVLHSRLSQCPEVHPLPTPVLLPAVDFSLGEWKTQMEETKAQDILGAVTLLLEGVMAARGQLGPTCLSSLLGQLSGQVRLLLGALQSLLGTQLPPQGRTTAHKDPNAIFLSFQHLLRGKVRFLMLVGGSTLCVRRAPPTTAVPSRTSLVLTLNEL
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofhumanMO7ecellswasfoundtobe1.0 ng/ml,correspondingtoaspecificactivityof1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof<0.5 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
