Type
Recombinant
Description
TNF-βisapotentmediatorofinflammatoryandimmuneresponses.ItbelongstotheTNFfamilyofligands,andsignalsthroughTNFR1andTNFR2.TNF-βisproducedbyactivatedTandBlymphocytes,andhassimilaractivitiestoTNF-α.LikeTNF-α,TNF-βisinvolvedintheregulationofvariousBIOLOGicalprocesses,includingcellproliferation,differentiation,apoptosis,lipidmetabolism,coagulation,andneurotransmission.TNF-βissecretedasasolublepolypeptide,butcanformheterotrimerswithlymphotoxin-β,whicheffectivelyanchorstheTNF-βtothecellsurface.TNF-βiscytotoxictoawiderangeoftumorcells.RecombinantHumanTNF-βisasecreted172 aminoacidprotein(18.6 kDa)whichformsanactivenon-disulfide-linkedhomotrimerstructureinsolution.
AminoAcidSequence
MLPGVGLTPSAAQTARQHPKMHLAHSTLKPAAHLIGDPSKQNSLLWRANTDRAFLQDGFSLSNNSLLVPTSGIYFVYSQVVFSGKAYSPKATSSPLYLAHEVQLFSSQYPFHVPLLSSQKMVYPGLQEPWLHSMYHGAAFQLTQGDQLSTHTDGIPHLVLSPSTVFFGAFAL
Source
E.coli
Purity
98%
BiologicalActivity
TheED50 asdeterminedbythecytolysisofmurineL929cellsinthepresenceofActinomycin-Dis≤0.05 ng/ml,correspondingtoaspecificactivityof≥2×107 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
