Type
Recombinant
Description
TNF-αisapleiotropicpro-inflammatorycytokinesecretedbyvariouscells,includingADIpocytes,activatedmonocytes,macrophages,Bcells,Tcellsandfibroblasts.ItbelongstotheTNFfamilyofligands,andsignalsthroughtworeceptors,TNFR1andTNFR2.TNF-αiscytotoxictoawidevarietyoftumorcells,andisanessentialfactorinmediatingtheimmuneresponseagainstbacterialinfections.TNF-αalsoplaysaroleintheinductionofsepticshock,autoimmunediseases,rheumatoidarthritis,inflammation,anddiabetes.HumanandmurineTNF-αdemonstratesignificantcross-speciesreactivity.TNF-αexistsintwoforms;atypeIItransmembraneprotein,andamaturesolubleprotein.TheTNF-αtransmembraneproteinisproteolyticallycleavedtoyieldasoluble,BIOLOGicallyactive,17 kDaTNF-α,whichformsanon-covalentlylinkedhomotrimerinsolution.RecombinantRatTNF-αisasoluble157 aminoacidprotein(17.3 kDa)whichcorrespondstoC-terminalextracellulardomainofthefulllengthtransmembraneprotein.
AminoAcidSequence
MLRSSSQNSSDKPVAHVVANHQAEEQLEWLSQRANALLANGMDLKDNQLVVPADGLYLIYSQVLFKGQGCPDYVLLTHTVSRFAISYQEKVSLLSAIKSPCPKDTPEGAELKPWYEPMYLGGVFQLEKGDLLSAEVNLPKYLDITESGQVYFGVIAL
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbythecytolysisofmurineL929cellsinthepresenceofactinomycinDis≤0.05 ng/ml,correspondingtoaspecificactivityof≥2×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
