Type

Recombinant

Description

TumorNecrosisFactor-aRatRecombinantproducedinE.Coliisasingle,non-glycosylated,polypeptidechaincontaining157 aminoacidsandhavingamolecularmassof17339.44 Dalton.TheTNF-alphaispurifiedbystandardchromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Leu-Arg-Ser-Ser.

Source

E.coli

Purity

Greaterthan97.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheED50asdeterminedbythecytolysisofmurineL929cellsinthepresenceofActinomycinDis<0.05 ng/ml,correspondingtoaSpecificActivityof5×107 IU/mg.

Formulation

Theproteinwaslyophilizedwithnoadditives.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedTumorNecrosisFactor-aalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionTNF-ashouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.