Type
Recombinant
Description
TLR-3 isasingle-passtypeI receptorthatbindstoandsignalsthepresenceofmicrobialpathogensanddoublestrandedRNA(dsRNA)viruses.SignalingthroughTLR-3 canpromotetheNF-κBpathwaytoinitiateinnateandadaptiveimmuneresponsestobacterialandviralinfections,aswellasthep53pathwaytotriggerapoptosisincellsinfectedwithdsRNAviruses.TLR-3 belongstoafamilyofstructurally-relatedtoll-likereceptors(TLRs)containinganN-terminaldomainrichinleucinerepeats,andaC-terminalintracellularToll/interleukin(IL)-1(TIL)domain.TLR-3 isexpressedprimarilyindendriticcellsoftheplacentaandpancreaswhereitcanresideonbothsidesoftheplasmamembrane,andintheendosomalcompartmentofthecells.RecombinantHumanTLR-3 is77.4 kDaglycoproteincontaining681 residueswhichcomprisetheTLR-3 extracellulardomain.
AminoAcidSequence
STTKCTVSHEVADCSHLKLTQVPDDLPTNITVLNLTHNQLRRLPAANFTRYSQLTSLDVGFNTISKLEPELCQKLPMLKVLNLQHNELSQLSDKTFAFCTNLTELHLMSNSIQKIKNNPFVKQKNLITLDLSHNGLSSTKLGTQVQLENLQELLLSNNKIQALKSEELDIFANSSLKKLELSSNQIKEFSPGCFHAIGRLFGLFLNNVQLGPSLTEKLCLELANTSIRNLSLSNSQLSTTSNTTFLGLKWTNLTMLDLSYNNLNVVGNDSFAWLPQLEYFFLEYNNIQHLFSHSLHGLFNVRYLNLKRSFTKQSISLASLPKIDDFSFQWLKCLEHLNMEDNDIPGIKSNMFTGLINLKYLSLSNSFTSLRTLTNETFVSLAHSPLHILNLTKNKISKIESDAFSWLGHLEVLDLGLNEIGQELTGQEWRGLENIFEIYLSYNKYLQLTRNSFALVPSLQRLMLRRVALKNVDSSPSPFQPLRNLTILDLSNNNIANINDDMLEGLEKLEILDLQHNNLARLWKHANPGGPIYFLKGLSHLHILNLESNGFDEIPVEVFKDLFELKIIDLGLNNLNTLPASVFNNQVSLKSLNLQKNLITSVEKKVFGPAFRNLTELDMRFNPFDCTCESIAWFVNWINETHTNIPELSSHYLCNTPPHYHGFPVRLFDTSSCKDSAPFEL
Source
HEK293cells
Purity
95%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibitIL-8 secretioninducedbyPolyI:CintheTLR3receptor-expressingHEK293cells(HEK293TLR3r).TheED50forthiseffectis30 µg/mlinthepresenceof100 µg/mlofPoly I:C.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
