Type

Recombinant

Description

TIMP-1 isanextracellularinhibitorofMMPs,includingMMP-1, –2, –3, –7, –8, –9, –10, –11, –12, –13,and –16.ItbelongstotheI35(TIMP)familyofirreversIBLeproteaseinhibitorsthatfunctionaskeymodulatorsofextracellularmatrixdegradationduringtissuedevelopmentandremodeling.TIMP-1 canalsoactthroughanMMP-independentmechanismtopromoteerythropoiesisbystimulatingproliferationanddifferentiationoferythroidProgenitors.RecombinantHumanTIMP-1 isa20.6 kDaproteincontaining184 aminoacidresidues.

AminoAcidSequence

CTCVPPHPQTAFCNSDLVIRAKFVGTPEVNQTTLYQRYEIKMTKMYKGFQALGDAADIRFVYTPAMESVCGYFHRSHNRSEEFLIAGKLQDGLLHITTCSFVAPWNSLSLAQRRGFTKTYTVGCEECTVFPCLSIPCKLQSGTHCLWTDQLLQGSEKGFQSRHLACLPREPGLCTWQSLRSQIA

Source

E.coli

Purity

95%

BIOLOGicalActivity

TIMP-1 activitywasmeasuredbyitsABIlitytoinhibithumanMMP-1-inducedhydrolysisofachromogenicpeptidesubstrateatroomtemperature.HalfmaximalinhibitionwasobtainedataTIMP-1 concentrationofapproximately0.5 μg/ml,whenusinganMMP-1 concentrationof1.6 μg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C