Type
Recombinant
Description
TIGARisap53-inducIBLeenzymethatcatalyzesthehydrolysisoffructose-2–6bisphosphate(F-2–6-BP)tofructose-6-phosphateandinorganicphosphate.F-2–6-BPisapowerfulactivatorof6-phosphofructose-1 kinase,theratelimitingenzymeofglycolysis.ByloweringtheintracellularlevelofF-2–6-BP,TIGARexpressionleadstoincreasedglucoseprocessingviathepentosephosphatepathway,themajorcellularsourceforNADPH.RecombinantHumanTIGARexpressedinE.coliisa29.9 kDaproteincontaining269 aminoacidresidues.
AminoAcidSequence
ARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR
Source
E.coli
Purity
95%
BIOLOGicalActivity
Datanotavailable.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C