Type
Recombinant
Description
Total120 AA.Mw:13.34 kDa(calculated).UniProtKBacc.no.Q86YW7.N-terminalHis-tag(14AA-highlighted).
AminoAcidSequence
MRGSHHHHHHGMASASSGNLRTFVGCAVREFTFLAKKPGCRGLRITTDACWGRCETWEKPILEPPYIEAHHRVCTYNETKQVTVKLPNCAPGVDPFYTYPVAIRCDCGACSTATTECETI
Source
E.coli
Purity
>95%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanThyrostimulinbetasubunit
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin0.05MAcetatebufferpH 4
Reconstitution
Add0.1MAcetatebufferpH4toprepareaworkingstocksolutionofapproximately0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.ForconversionintohigherpHvalue,werecommendintensivedilutionbyrelevantbuffertoaconcentrationof10μg/mL.Inhigherconcentrationsthesolubilityofthisantigenislimited.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
Note
Thisproductisintendedforresearchuseonly.