Type
Recombinant
Description
TGF-αisanEGF-relatedpolypeptidegrowthfactorthatsignalsthroughtheEGFreceptor,andstimulatestheproliferationofawiderangeofepidermalandepithelialcells.Itisproducedbymonocytes,keratinocytes,andvarioustumorcells.TGF-αinducestransformationanchorageindependenceinculturedcells.Human,murineandratTGF-αarecross-speciesreactive.RecombinanthumanTGF-αisa50 aminoacidpolypeptide(5.5 kDa)whichsharesapproximately40%sequencehomologywithEGF,including6 conservedcysteineresidues,whichform3 intramoleculardisulfide bonds.
AminoAcidSequence
VVSHFNDCPDSHTQFCFHGTCRFLVQEDKPACVCHSGYVGARCEHADLLA
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 asdeterminedbythedose-dependentstimulationofthymidineuptakebyBALB/c3T3cellsis≤0.2 ng/ml,correspondingtoaspecificactivityof≥5×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
