Type
Recombinant
Description
Total141 AA.MW:16.2 kDa(calculated).UniProtKBacc.no.Q0Z7S8.N-TerminalHis-tag(10 extra AA)
AminoAcidSequence
MKHHHHHHASVEPFLGTWKLVSSENFEDYMKELGVNFAARNMAGLVKPTVTISVDGKMMTIRTESSFQDTKISFKLGEEFDETTADNRKVKSTITLENGSMIHVQKWLGKETTIKRKIVDEKMVVECKMNNIVSTRIYEKV
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis > 99%
SDS-PAGEGel
14%SDS-PAGEseparationofHumanFABP9
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5
Reconstitution
Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.
Applications
Westernblotting,ELISA
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4°Cfora week.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.