Type

Recombinant

Description

TACI,amemberoftheTNFReceptorsuperfamily,isexpressedinthesmallintestine,spleen,thymus,peripheralbloodleukocytes,activatedTcells,andrestingBcells.TACIcanbindtobothAPRILandBAFF,stimulatetheactivationoftranscriptionfactorsNF-κBandAP-1,andcanmediatethecalcineurin-dependentactivationofNF-AT(nuclear-factorofactivatedTcells).TACIalsoplaysakeyroleinthestimulationofBandTcellfunction.SolubleTACIinhibitsAPRIL-stimulatedproliferationofprimaryBcellsbyblockingthebindingofAPRILtothemembrane-anchoredTACIreceptor.RecombinantHumanTACIisasoluble159 aminoacidpolypeptide(17.8 kDa)comprisingtheTNFRhomologous,cysteine-richextracellulardomainoftheTACIprotein.

AminoAcidSequence

SGLGRSRRGGRSRVDQEERFPQGLWTGVAMRSCPEEQYWDPLLGTCMSCKTICNHQSQRTCAAFCRSLSCRKEQGKFYDHLLRDCISCASICGQHPKQCAYFCENKLRSPVNLPPELRRQRSGEVENNSDNSGRYQGLEHRGSEASPALPGLKLSADQV

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoblockhumanBAFFinducedT2Bcellsurvivalusingaconcentrationrangeof1.0–3.0 µg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C