Type
Recombinant
Description
TRAIL/Apo2Lisacytotoxicprotein,whichactivatesrapidapoptosisintumorcells,butnotinnormalcells.TRAIL-inducedapoptosisisachievedthroughbindingtotwodeath-signalingreceptors,DR4andDR5.ThesereceptorsbelongtotheTNFRsuperfamilyoftransmembraneproteins,andcontainacytoplasmic“deathdomain”,whichactivatesthecell"s apoptoticmachinery.ThefulllengthhumanTRAIL/Apo2Lisa281 aminoacidprotein,consistingofa17 aminoacidcytoplasmicdomain,a21 aminoacidtransmembranedomain,anda243 aminoacidextracellulardomain.RecombinantHumansolubleTRAIL/Apo2Lisa168 aminoacidpolypeptide(19.6 kDa),consistingoftheTNF-homologousportionoftheextracellulardomainofthefulllengthTRAIL/Apo2Lprotein.
AminoAcidSequence
MRERGPQRVAAHITGTRGRSNTLSSPNSKNEKALGRKINSWESSRSGHSFLSNLHLRNGELVIHEKGFYYIYSQTYFRFQEEIKENTKNDKQMVQYIYKYTSYPDPILLMKSARNSCWSKDAEYGLYSIYQGGIFELKENDRIFVSVTNEHLIDMDHEASFFGAFLVG
Source
E.coli
Purity
98%
BIOLOGicalActivity
Assay#1:DeterminedbyitsABIlitytoinduceapoptoticcelldeathinTRAIL-sensitiveU343MGcells.TheexpectedED50forthiseffectis1.0–3.0 ng/ml.Assay#2:MeasuredbyitsabilitytoinduceapoptosisinLN-18 cells(humanglioblastomacells).TheexpectedED50forthiseffectis0.8 –2.0 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
