Type
Recombinant
Description
TRAILReceptor-1/DR4andTRAILReceptor-2/DR5belongtotheTNFRsuperfamilyoftransmembraneproteins,andcontainacytoplasmic“deathdomain,”whichcanactivatethecell"s apoptoticmachinery.Thesereceptorsareactivatedbybindingtoeithermembrane-anchoredorsolubleTRAIL/Apo2L.TheDR4andDR5receptorsarebothproducedastypeI transmembraneproteins,whichcontainanextracellulardomain,atransmembranedomain,andacytoplasmicdomain.TherecombinantsolubleformsofDR4andDR5consistoftheTNFR-homologous,cysteine-richportionoftheirrespectiveextracellulardomains.RecombinantHumansolubleTRAILReceptor-1/DR4isa22.7 kDaprotein(215 aminoacidresidues)consistingoftheTNFRhomologous,cysteine-richportionoftheextracellulardomain.
AminoAcidSequence
MSGTGAAAATPSKVWGSSAGRIEPRGGGRGALPTSMGQHGPSARARAGRAPGPRPAREASPRLRVHKTFKFVVVGVLLQVVPSSAATIKLHDQSIGTQQWEHSPLGELCPPGSHRSERPGACNRCTEGVGYTNASQQLFACLPCTACKSDEEERSPCTTTRNTACQCKPGTFRNDNSAEMCRKCSTGCPRGMVKVKDCTPWSDIECVHKESGNGHN
Source
E.coli
Purity
98%
BIOLOGicalActivity
MeasuredbyitsABIlitytoinhibitapoptosisinLN-18 cells.TheexpectedED50forthiseffectis0.4 –0.5 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
