Type
Recombinant
Description
TNFRIIisamemberoftheTNFRfamilyoftransmembraneproteins,andisexpressedinimmunecellsandcertainendothelialcells.ItisahighaffinityreceptorforTNF-α,butmanifestsaloweraffinitytoTNF-β.SignalingthroughthisreceptorregulatesvariousBIOLOGicalprocesses,includingcellproliferation,differentiation,apoptosis,lipidmetabolism,coagulation,andneurotransmission.SolubleTNFRIIiscapableofinhibitingTNF-α-inducedactivitiesbyactingasadecoyreceptor.ThehumanTNFRIIgeneencodesfora461 aminoacidtypeI transmembraneprotein,whichcontainsa22 aminoacidsignalsequence,a235 aminoacidextracellulardomain,a30 aminoacidtransmembranedomain,anda174 aminoacidcytoplasmicdomain.RecombinantHumansTNFReceptorTypeIIisan18.9 kDaprotein(174 aminoacidresidues)comprisingthecysteine-rich,ligandbindingportionoftheextracellulardomainoftheTNFRIIprotein.
AminoAcidSequence
MAPEPGSTCRLREYYDQTAQMCCSKCSPGQHAKVFCTKTSDTVCDSCEDSTYTQLWNWVPECLSCGSRCSSDQVETQACTREQNRICTCRPGWYCALSKQEGCRLCAPLRKCRPGFGVARPGTETSDVVCKPCAPGTFSNTTSSTDICRPHQICNVVAIPGNASMDAVCTSTSP
Source
E.coli
Purity
98%
BiologicalActivity
DeterminedbyitsinhibitoryeffectoftheTNF-αmediatedcytotoxicityinmurineL-929 cells. TheED50forthiseffectinthepresenceof0.25 ng/mlofrecombinanthumanTNF-α,is0.125 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
