Type
Recombinant
Description
TNFRIbelongstotheTNFRsuperfamilyoftransmembraneproteins,andisexpressedinmostcelltypes.BindingofeitherTNF-αorTNF-βtoTNFRIinitiatesasignaltransductionpathwaythatresultsintheactivationofthetransductionfactorNF-κB,whosetargetgenesareinvolvedintheregulationofinflammatoryresponses,and,incertaincellsinduceapoptosis.SolubleTNFReceptorI(sTNFRI)iscapableofinhibitingTNF-αandTNF-βactivitiesbyactingasadecoyreceptorthatservesasasinkfortheTNFligands.ThehumanTNFRIgeneencodesfora455 aminoacidtypeI transmembraneprotein,whichcontainsa21 aminoacidsignalsequence,a190 aminoacidextracellulardomain,a23 aminoacidtransmembranedomain,anda221 aminoacidcytoplasmicdomain.RecombinantHumansTNFReceptorTypeI isan18.3 kDaprotein(162 aminoacidresidues)comprisingthecysteine-rich,ligand-bindingportionoftheextracellulardomainoftheTNFRIprotein.
AminoAcidSequence
MDSVCPQGKYIHPQNNSICCTKCHKGTYLYNDCPGPGQDTDCRECESGSFTASENHLRHCLSCSKCRKEMGQVEISSCTVDRDTVCGCRKNQYRHYWSENLFQCFNCSLCLNGTVHLSCQEKQNTVCTCHAGFFLRENECVSCSNCKKSLECTKLCLPQIEN
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsinhibitoryeffectoftheTNF-αmediatedcytotoxicityinmurineL-929 cells.TheED50forthiseffectinthepresenceof0.25 ng/mlofrecombinanthumanTNF-αis0.05 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
