Type
Recombinant
Description
RecombinantHumanSCFproducedinE.Coliisasingle,non-glycosylatedpolypeptidechaincontaining164 aminoacidsandhavingamolecularmassof18.4 kDa.TheSCFispurifiedbyproprietarychromatographictechniques.
AminoAcidSequence
ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Glu-Gly-Ile-Cys.
Source
E.coli
Purity
Greaterthan98.0%asdeterminedby:-(a)AnalysisbyRP-HPLC.-(b)Anion-exchangeFPLC.-©Analysisbyreducingandnon-reducingSDS-PAGESilverStained gel.
BIOLOGicalActivity
Biovendor´s recombinanthumanSCFisfullybiologicallyactivewhencomparedtostandard.TheED50asdeterminedbythedose-dependantstimulationofHumanTF-1 cellsis<2 ng/ml,correspondingtoaSpecificActivityof5×105 IU/mg.
Endotoxin
Lessthan0.1 ng/Cg(IEU/Cg)ofrHuSCF.
Formulation
Lyophilizedfromaconcentrated(1mg/ml)solutioninwatercontaining10mMAcetic acid.
Shipping
Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
LyophilizedrHuSCFalthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionrHuSCFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.
PhysicalAppearance
Sterilefilteredwhitelyophilized(freeze-dried)powder.
